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植物甾醇油酸酯的制备及其在负载 虾青素的脂质体中的应用Preparation of phytosterol oleate and its application in astaxanthin-loaded liposomes
常振刚1,潘丽2,张新2,谷克仁2,田一凡2 CHANG Zhengang1, PAN Li2, ZHANG Xin2, GU Keren2, TIAN Yifan2
Zhongguo you zhi, 2022-11, Vol.47 (11), p.92-97
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題名:
植物甾醇油酸酯的制备及其在负载 虾青素的脂质体中的应用Preparation of phytosterol oleate and its application in astaxanthin-loaded liposomes
著者:
常振刚1,潘丽2,张新2,谷克仁2,田一凡2 CHANG Zhengang1, PAN Li2, ZHANG Xin2, GU Keren2, TIAN Yifan2
主題:
phytosterol esters
;
oleic acid
;
astaxanthin
;
complex liposomes
;
植物甾醇酯;油酸;虾青素;复合脂质体
所屬期刊:
Zhongguo you zhi, 2022-11, Vol.47 (11), p.92-97
描述:
为了探索植物甾醇酯构建脂质体的可行性,以植物甾醇和油酸为原料合成植物甾醇油酸酯并进行分离纯化,运用红外光谱法和核磁共振法对植物甾醇油酸酯进行表征。以大豆磷脂为主要膜材,运用薄膜-超声法构建虾青素-植物甾醇油酸酯复合脂质体,并对其粒径大小及分布、Zeta电位、包封率以及微观形貌性质进行研究。结果表明:制备的植物甾醇油酸酯经分离纯化后纯度为(96.51±0.93)%,构建的虾青素-植物甾醇油酸酯复合脂质体近似球形,形状规则且分散性好,平均粒径为(124.0±12.5)nm,多相分散系数(PDI)为0.37±0.01,平均Zeta电位为(-33.3±1.6)mV,包封率为(93.95±0.92)%。综上,采用植物甾醇油酸酯负载虾青素,包封率高,制备的复合脂质体稳定,分布均一,说明植物甾醇油酸酯可应用于负载脂溶性生物活性物质的脂质体的构建。 In order to explore the feasibility of preparing liposomes by phytosterol esters, using phytosterol and oleic acid as materials, phytosterol oleate was prepared, separated and structure characterized by infrared spectroscopy and nuclear magnetic resonance. The complex liposomes of astaxanthin-phytosterol oleate were prepared by using soy phospholipids as the main membrane material by a thin-film ultrasound method,and its particle size and distribution, Zeta potential, encapsulation efficiency, and morphology were studied. The results showed the purity of phytosterol oleate was (96.51±093)% after purification. The prepared astaxanthin-phytosterol oleate complex liposomes were nearly spherical with regular shape and good dispersion, and the average particle size was (124.0±12.5) nm with the polydispersity index (PDI) of 0.37±0.01, the average Zeta potential was (-33.3±1.6) mV and the encapsulation efficiency was (93.95±0.92)%. In conclusion, using phytosterol oleate for loading astaxanthin, the encapsulation rate is high and the prepared complex liposomes are stable and uniformly distributed, and the phytosterol oleate can be applied to the construction of liposomes loaded with lipid-soluble bioactive substances.
出版者:
中粮工科(西安)国际工程有限公司
語言:
英文
識別號:
ISSN: 1003-7969
DOI: 10.19902/j.cnki.zgyz.1003-7969.220278
資源來源:
DOAJ Directory of Open Access Journals
Alma/SFX Local Collection
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