skip to main content
訪客
個人書架
我的帳戶
登出
登入
This feature requires javascript
檢索首頁
圖書館首頁
電子期刊
引用參考文獻查詢
指定參考書查詢
新書通報
標籤查詢
線上輔助
語言:
English
繁體中文
This feature required javascript
This feature requires javascript
Primo Search
館藏+文章
館藏+文章
館藏
查館藏
文章
查文章
機構典藏
機構典藏
Search For:
Clear Search Box
Search in:
館藏
Or hit Enter to replace search target
Or select another collection:
Search in:
館藏
進階檢索
瀏覽查詢
This feature requires javascript
資源種類
criteria input
圖書
期刊
視聽資料
全部館藏
顯示結果:
criteria input
包含在我的檢索語句內
完全相同
起始以
顯示結果:
查詢種類 索引
criteria input
任何地方
題名
ISBN
ISSN
Show Results with:
題名
Show Results with:
任何地方
題名
ISBN
ISSN
This feature requires javascript
Measuring and modeling enhancers in perturbed Drosophila melanogaster embryos.
Max Valentin. Staller Harvard University. Systems Biology. 2015
Dissertation Abstracts International 76-09B(E).
線上取得
線上檢視
詳細格式
評論和標籤
相關文章推薦
FullText@NUTN
This feature requires javascript
傳送到
加入個人書架
從個人書架中移除
E-mail
列印
永久連結
引用
EndNote
導出 RiS
This feature requires javascript
題名:
Measuring and modeling enhancers in perturbed Drosophila melanogaster embryos.
著者:
Max Valentin. Staller
Harvard University. Systems Biology.
主題:
Genetics
所屬期刊:
Dissertation Abstracts International 76-09B(E).
描述:
The diversity of animal shapes and sizes, colors and textures, or behaviors and habitats all depend on specialized cells. A newly fertilized embryo must build all these specialized cell types, a process called differentiation. Much of differentiation depends on appropriately turning genes on and off in each cell type. Cell type specific control of gene expression is encoded in a type of regulatory DNA called enhancers. I am interested in how enhancers control the cell type specific gene expression that enables specialized cell functions.
Enhancers read in information from regulatory proteins and output a level of gene expression. This conversion from input regulator concentrations to output expression level is a computation. I use quantitative measurement and computational modeling to study how enhancers compute. In embryos, many regulatory proteins bind to enhancers, and some will turn an enhancer on, while others will turn it off. This complex process is greatly simplified by employing computational models. These models can test whether all regulators have been identified (and if not, find the missing ones) and quantify the relationships between regulators. The relationships between regulators reflect the underlying molecular mechanisms used in the cell; when several models can fit the data, perturbation experiments can be used to distinguish the models and underlying mechanisms. However, most computational models of gene expression in animals have not been rigorously validated by perturbation experiments. A major contribution of my thesis work was developing methods for testing models.
To test computational models for how enhancers compute gene expression patterns, I experimentally manipulated the concentrations of regulatory proteins and precisely measured output gene expression patterns. Using the Drosophila melanogaster blastoderm embryo, I first developed efficient and scalable techniques for making perturbations to regulatory protein concentrations. This technique revealed a postulated property of development: that embryos mitigate the impact of perturbations by preventing the creation of new cell types. I then used two perturbations to test computational models of an enhancer, finding they were incomplete and discovering new regulatory connections. My work illustrates how computational modeling and quantitative measurement are powerful tools for untangling how regulatory DNA operates in embryos.
出版者:
Thesis (Ph.D.)--Harvard University, 2015.
建立日期:
2015
格式:
194 p..
語言:
英文
識別號:
ISBN9781321705492
資源來源:
NUTN ALEPH
連結
連結圖書館目錄
Electronic resource
連結到 Amazon.com
連結到 WorldCat®
This feature requires javascript
This feature requires javascript
返回到檢索清單
This feature requires javascript
This feature requires javascript
正在檢索遠程資料庫,請稍等
查詢:
在
scope:("NUTN"),scope:(NUTN_ALEPH)
顯示現有記錄
This feature requires javascript
This feature requires javascript