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DOPA DECARBOXYLASE(DE1), A MUTATION DIFFERENTIALLY AFFECTING BOTH STAGE AND TISSUE SPECIFIC EXPRESSION OF DOPA DECARBOXYLASE IN DROSOPHILA.

CLIFTON PATTON. BISHOP University of Virginia. 1986

Dissertation Abstracts International 48-02B.

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DOPA DECARBOXYLASE(DE1), A MUTATION DIFFERENTIALLY AFFECTING BOTH STAGE AND TISSUE SPECIFIC EXPRESSION OF DOPA DECARBOXYLASE IN DROSOPHILA.
著者: CLIFTON PATTON. BISHOP
University of Virginia.
主題: Genetics
所屬期刊: Dissertation Abstracts International 48-02B.
描述: The isolation and characterization of a unique Dopa decarboxylase (Ddc) mutant, Ddc('DE1), in Drosophila melanogaster is reported. Homozygous Ddc('DE1) embryos, central nervous systems at pupariation and newly eclosed adult epidermis all have approximately 5% the specific dopa decarboxylase (DDC) activity of controls while the epidermis at pupariation has roughly 50% as much DDC activity. Although the adult cuticle lacks proper pigmentation, the bristles unexpectedly have wild type black pigmentation, suggesting the bristle forming cells have more DDC activity than the rest of the adult epidermis.
This variegated phenotype, black bristles and pale cuticle, plus the fact that Ddc('DE1) was originally isolated in a reciprocal translocation between proximal X heterochromatin and the euchromatic left arm of the second chromosome suggested that the mutant phenotype might be due to position-effect variegation. All tests for position-effect variegation, including persistance of the mutant phenotype when Ddc('DE1) was removed from the translocation, were negative.
The lesion in Ddc('DE1) maps genetically to the Ddc locus and although it was (gamma)-ray induced, extensive Southern analysis of Ddc('DE1) DNA failed to detect a change in the DNA. Embryonic DDC RNA levels are reduced in Ddc('DE1) relative to wild type and there is no build up of unprocessed RNAs in the mutant.
Ddc('DE1) has more DDC cross reacting material at both pupariation and adult eclosion than the DDC specific activity would suggest. DDC from both Ddc('DE1) white prepupae and newly eclosed adults is more thermolabile than wild type DDC at these stages. These results indicate Ddc('DE1) produces a defective DDC enzyme at both stages. Thermolability studies suggested that two forms of DDC are present in wild type at different stages.
Ddc('DE1) hemizygotes have less than half the DDC activity of homozygotes. Evidence to support the idea that this is due to altered kinetics of DDC enzyme formation is presented.
Two theories to explain the differential stage and tissue specific DDC activities observed in Ddc('DE1) are presented and discussed.
出版者: Thesis (Ph.D.)--University of Virginia, 1986.
建立日期: 1986
格式: 140 p..
語言: 英文
資源來源: NUTN ALEPH

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DOPA DECARBOXYLASE(DE1), A MUTATION DIFFERENTIALLY AFFECTING BOTH STAGE AND TISSUE SPECIFIC EXPRESSION OF DOPA DECARBOXYLASE IN DROSOPHILA.

CLIFTON PATTON. BISHOP University of Virginia. 1986

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