耐力訓練與熱量限制對母鼠骨骼肌質量及能量代謝酵素之影響

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題名:
耐力訓練與熱量限制對母鼠骨骼肌質量及能量代謝酵素之影響
著者: 張書瑋; Chang, Shu-wei
劉立宇; Li-yu Liu; 體育學系碩士班
主題: 能量代謝; 熱量限制; 耐力訓練; 酵素; energy metabolism; calorie restriction; endurance training; enzyme
描述: 目的：本研究旨在探討8週運動訓練與熱量限制對母鼠骨骼肌重量及骨骼肌能量代謝酵素之影響。方法：以雌性Wistar大鼠48隻（週齡：7週）為研究對象，隨機分成四組（每組n＝12），分別為：低強度(70% VO2max)運動組（LIT組）、高強度(85% VO2max)運動組（HIT組）、控制組（CON組）及飲食控制組（DCON組）。運動組進行為期8週，每週5天，每天60分鐘的跑步機運動訓練；在熱量限制方面，每日給予DCON組的飼料為運動組平均飲食量之80%至90%。除了每週記錄二次體重外，每組皆在實驗後測量肌肉重及檸檬酸合成酶(CS)、3-羥醯基輔酶A脫氫酶(β-HAD)、乳酸脫氫酶(LDH)及磷酸果糖激酶(PFK)活性。所得數據以單因子變異數分析比較各組的體重、肌肉重及各種酵素的活性。結果：第二週開始DCON組母鼠的體重在顯著低於HIT組、LIT組與CON組（p<.05），並且維持到第八週。而在肌肉重量方面，比目魚肌(SOL)的重量DCON組顯著低於其他三組（p<.05）；伸趾長肌(EDL)重量DCON組顯著低於LIT組與HIT兩組。在骨骼肌能量代謝酵素方面，SOL的CS、β-HAD、LDH及PFK活性組間皆無顯著差異；EDL的CS、β-HAD、PFK活性組間亦皆無顯著差異，但是LDH活性則為DCON組高於HIT組（p<.05）。結論：熱量限制可有效減緩母鼠體重上升。此外，耐力運動無助於骨骼肌中能量代謝酵素活性的增加，而熱量限制則會使EDL的LDH活性提升。
Purpose：This study investigated the effect of endurance training and calorie restriction on skeletal muscle mass and energy metabolism in female rats for 8 weeks. Method：48 female Wistar rats（7 weeks）were randomly divided into four groups（n＝12 in each group）：low intensity (70% VO2max) endurance training（LIT）, high intensity (85% VO2max) endurance training（HIT）, control group（CON） and diet control group（DCON）. Exercise groups received treadmill training for 8 weeks：60 minutes a day and 5 days a week. For calorie restriction, DCON group were daily fed 80%-90% of exercise groups. Weights were recorded twice a week. Measured muscle weight and assayed activity of citrate Synthase (CS), 3-hydroxyacyl-CoA dehydrogenase (β-HAD), lactate dehydrogenase (LDH) and phosphofructokinase (PFK) after experiment for each group. Recorded data was analyzed by One-way analysis of variance (ANOVA) to compare weight, muscle weight and enzyme activity. Results：DCON group was significantly lower than HIT group, LIT group and CON group（p<.05）after 2th weeks. For muscle weight, Soleus (SOL) weight of DCON group was significantly lower than other 3 groups（p<.05）；Extensor digitorum longus (EDL) weight of DCON group was significantly lower than LIT and HIT groups. On the part of skeletal muscle energy metabolism enzyme, there was no significant effect in CS, β-HAD, LDH and PFK activity of SOL, and no significant effect in CS, β-HAD and PFK activity of EDL, either. DCON group is higher than HIT group（p<.05）in LDH activity. Conclusion：Calorie restriction lower weight in female rats. In addition, there wasn’t significant effect in endurance training to increase energy metabolism enzyme activity in skeletal muscle. However, calorie restriction increased LDH activity in EDL.
碩士
建立日期: 2011
格式: 121 bytes
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語言: 中文
識別號: http://nutnr.lib.nutn.edu.tw/handle/987654321/1878
資源來源: NUTN IR

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耐力訓練與熱量限制對母鼠骨骼肌質量及能量代謝酵素之影響

張書瑋; Chang, Shu-wei 劉立宇; Li-yu Liu; 體育學系碩士班 2011

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