利用稻田魚篩選對魚類病毒性神經壞死症具抗病性之中草藥研究

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題名:
利用稻田魚篩選對魚類病毒性神經壞死症具抗病性之中草藥研究
著者: 劉佩雯; Liu, Pei-wen
曾登裕; none; 生物科技學系碩士班
主題: 神經壞死病毒; 稻田魚; NNV; Nervous necrosis virus; medaka
描述: 神經壞死病毒（Nervous Necrosis Virus, NNV），在台灣引起許多種海水養殖魚苗的高死亡率。本研究目的先進行宿主感受性測試以決定其攻毒試驗劑量，再以中草藥萃取物，進行抗神經壞死症病毒物質分析。首先利用稻田魚對於grouper nervous necrosis virus（GNNV）的感受性實驗，將稻田魚孵化後2 日之幼苗，於5ml 曝氣水中養殖20 尾，各加入不同劑量之109 TCID50/ml 病毒液，結果以250μl 的劑量具有較佳之感受性，稻田魚幼苗感染三天後的死亡率超過一半，故以250μl 病毒液為攻毒劑量。在相同條件下，我們再測試許多種中草藥萃取物，將稻田魚剛孵化之幼苗，於5ml 曝氣水中養殖30 尾，實驗組先加入中草藥萃取液，控制組不加，養殖2 日後，各組均進行攻毒試驗，結果控制組在GNNV加入3 天後，死亡率超過一半，藥物實驗組死亡率極低，試驗的中草藥萃取物中有一種對於魚類感染GNNV 有最佳之抗病性，且在第五天的存活率與控制組十分接近。此稻田魚幼苗感染分析方法，未來可以作為抗神經壞死症病毒物質篩選分析平台，快速篩選具抗病毒之藥物。
Nervous necrosis virus (NNV) has caused a high death rate of numerous marine fish species at larval stage in Taiwan. The purpose of this study first is to test host susceptibility to determine the dose of, and then by using Chinese herbal extract to analyze anti-substance analysis nervous necrosis virus. We use medaka for grouper nervous necrosis virus (GNNV) the sensitivity experiments. The group used 20 fish seeding of medaka larvae hatched after 2 days in 5ml water, to be cultivated with different doses of 109 TCID50/ml virus solution; 250μl of dose resulted in with better sensitivity. The death rate that mekada seedlings are infected and die after3 days is more than half. So the amount of liquid of 250μl virus is used of attack agents. Under the same conditions, we re-tested many kinds of Chinese herbal extract, and succeeded in screening a Chinese herbal extract to anti-GNNV infection. The group used 30 fish seeding of medaka larvae in 5ml water , and then the experimental group poured the Chinese herbal extract into the water, compared with the control group without Chinese herbal extract 2 days later, each group had an attack drug test. The result is that for the control group after in the GNNV was poured into, the death rate was more than half；however, for the drug test group the death rate is very low, that is Chinese herbal extract , Galla Chinensis can effectively improve the survival rate of seedlings for GNNV infection. The next step is about the anti-virus analysis for other fish species by using Galla Chinensis. It is suggested that the analytic method of the infection of medaka larvae can be used as a platform to screen the anti-matter nervous necrosis virus to fast find out an anti-NNV drug in the future.
碩士
建立日期: 2012
格式: 121 bytes
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語言: 中文
識別號: http://nutnr.lib.nutn.edu.tw/handle/987654321/813
資源來源: NUTN IR

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利用稻田魚篩選對魚類病毒性神經壞死症具抗病性之中草藥研究

劉佩雯; Liu, Pei-wen 曾登裕; none; 生物科技學系碩士班 2012

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