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Phenotyping Thermal Responses of Yeasts and Yeast-like Microorganisms at the Individual and Population Levels: Proof-of-Concept, Development and Application of an Experimental Framework to a Plant Pathogen

Boixel, Anne-Lise ; Delestre, Ghislain ; Legeay, Jean ; Chelle, Michaël ; Suffert, Frédéric

Microbial ecology, 2019-07, Vol.78 (1), p.42-56 [同儕審閱期刊]

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題名:
Phenotyping Thermal Responses of Yeasts and Yeast-like Microorganisms at the Individual and Population Levels: Proof-of-Concept, Development and Application of an Experimental Framework to a Plant Pathogen
著者: Boixel, Anne-Lise ; Delestre, Ghislain ; Legeay, Jean ; Chelle, Michaël ; Suffert, Frédéric
主題: Adaptation ; Assessments ; Biodiversity ; Biological evolution ; Biomedical and Life Sciences ; Dimensions ; Dynamics ; Ecological monitoring ; Ecology ; ENVIRONMENTAL MICROBIOLOGY ; Epidemiology ; Fitness ; Foundations ; Frameworks ; Geoecology/Natural Processes ; Heterogeneity ; Life Sciences ; Micro-organisms ; Microbial Ecology ; Microbiology ; Microorganisms ; Nature Conservation ; Pathogens ; Phenotypes ; Phenotyping ; Population levels ; Protocols ; Sensitivity ; Sensitivity analysis ; Strains ; Strains (organisms) ; Temperature ; Temperature dependence ; Temperature effects ; Thermal environments ; Water Quality/Water Pollution ; Wheat ; Yeast ; Yeasts
所屬期刊: Microbial ecology, 2019-07, Vol.78 (1), p.42-56
描述: Deciphering the responses of microbial populations to spatiotemporal changes in their thermal environment is instrumental in improving our understanding of their eco-evolutionary dynamics. Recent studies have shown that current phenotyping protocols do not adequately address all dimensions of phenotype expression. Therefore, these methods can give biased assessments of sensitivity to temperature, leading to misunderstandings concerning the ecological processes underlying thermal plasticity. We describe here a new robust and versatile experimental framework for the accurate investigation of thermal performance and phenotypic diversity in yeasts and yeast-like microorganisms, at the individual and population levels. In addition to proof-of-concept, the application of this framework to the fungal wheat pathogen Zymoseptoria tritici resulted in detailed characterisations for this yeast-like microorganism of (i) the patterns of temperature-dependent changes in performance for four fitness traits; (ii) the consistency in thermal sensitivity rankings of strains between in planta and in vitro growth assessments; (iii) significant interindividual variation in thermal responses, with four principal thermotypes detected in a sample of 66 strains; and (iv) the ecological consequences of this diversity for population-level processes through pairwise competition experiments highlighting temperature-dependent outcomes. These findings extend our knowledge and ability to quantify and categorise the phenotypic heterogeneity of thermal responses. As such, they lay the foundations for further studies elucidating local adaptation patterns and the effects of temperature variations on eco-evolutionary and epidemiological processes.
出版者: New York: Springer Science + Business Media
語言: 英文
識別號: ISSN: 0095-3628
EISSN: 1432-184X
DOI: 10.1007/s00248-018-1253-6
PMID: 30280234
資源來源: SpringerLink Journals AutoHoldings
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Phenotyping Thermal Responses of Yeasts and Yeast-like Microorganisms at the Individual and Population Levels: Proof-of-Concept, Development and Application of an Experimental Framework to a Plant Pathogen

Boixel, Anne-Lise ; Delestre, Ghislain ; Legeay, Jean ; Chelle, Michaël ; Suffert, Frédéric

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